Ottawa Institute of Systems Biology

Publications from the laboratory of Daniel Figeys
	Improvement of the quantification accuracy and throughput for phosphoproteome analysis by a pseudo triplex stable isotope dimethyl labeling approach. Song C, Wang F, Ye M, Cheng K, Chen R, Zhu J, Tan Y, Wang H, Figeys D, Zou H. Anal Chem. 2011 Sep 8. [Epub ahead of print]
	Uncovering the proteome response of the master circadian clock to light using an AutoProteome system. Tian R, Cheng HY, Figeys D. Mol Cell Proteomics. 2011 Aug 22. [Epub ahead of print]
	Proteomic reactors and their applications in biology. Zhou H, Ning Z, Wang F, Seebun D, Figeys D. FEBS J. 2011 Aug 9. doi: 10.1111/j.1742-4658.2011.08292.x. [Epub ahead of print]
	An enhanced chemically defined SILAC culture system for quantitative proteomics study of human embryonic stem cells., Wang S, Tian R, Li L, Figeys D, Wang L., Proteomics. 2011 Jul 19. doi: 10.1002/pmic.201100052. [Epub ahead of print]
	Improved recovery and identification of membrane proteins from rat hepatic cells using a centrifugal proteomic reactor., Zhou H, Wang F, Yao Z, Wang Y, Ning Z, Hou W, Wright TG, Sundaram M, Zhong S, Figeys D., Mol Cell Proteomics. 2011 Jul 12.
	Missense mutation in APOC3 within the C-terminal lipid-binding domain of human apoC-III results in impaired assembly and secretion of triacylglycerol-rich very low density lipoproteins: Evidence that APOC-III plays a major role in the formation of lipid precursors within the microsomal lumen. Qin W, Sundaram M, Wang Y, Zhou H, Zhong S, Chang CC, Manhas S, Yao EF, Parks RJ, McFie PJ, Stone SJ, Jiang ZG, Wang C, Figeys D, Jia W, Yao Z. J Biol Chem. 2011 Jun 15. [Epub ahead of print]
	Skeletal myosin light chain kinase regulates skeletal myogenesis by phosphorylation of MEF2C. Al Madhoun AS, Mehta V, Li G, Figeys D, Wiper-Bergeron N, Skerjanc IS. EMBO J. 2011 May 10;30(12):2477-89.
	Development of a multiplexed microfluidic proteomic reactor and its application for studying protein-protein interactions. Tian R, Hoa XD, Lambert JP, Pezacki JP, Veres T, Figeys D. Anal Chem. 2011 Jun 1;83(11):4095-102.
	Analytical aspects of proteomics: 2009-2010. Ning Z, Zhou H, Wang F, Abu-Farha M, Figeys D. Anal Chem. 2011 Jun 15;83(12):4407-26.
	Srf1 is a novel regulator of phospholipase d activity and is essential to buffer the toxic effects of c16:0 platelet activating factor. Kennedy MA, Kabbani N, Lambert JP, Swayne LA, Ahmed F, Figeys D, Bennett SA, Bryan J, Baetz K. PLoS Genet. 2011 Feb 10;7(2):e1001299.
	Quantitative Proteomic Analysis of PCSK9 Gain of Function in Human Hepatic HuH7 Cells. Denis NJ, Palmer-Smith H, Elisma F, Busuttil A, Wright TG, Khalil MB, Prat A, Seidah NG, Chrétien M, Mayne J, Figeys D. J Proteome Res. 2011 Feb 18. [Epub ahead of print]
	Defining the budding yeast chromatin-associated interactome. Lambert JP, Fillingham J, Siahbazi M, Greenblatt J, Baetz K, Figeys D. Mol Syst Biol. 2010 Dec 21;6:448.PMID: 21179020
	Lyso-form fragment ions facilitate the determination of stereospecificity of diacyl glycerophospholipids. Hou W, Zhou H, Khalil MB, Seebun D, Bennett SA, Figeys D. Rapid Commun Mass Spectrom. 2011 Jan 15;25(1):205-17. doi: 10.1002/rcm.4846.PMID: 21157865
	The precursor to the germ cell-specific PCSK4 proteinase is inefficiently activated in transfected somatic cells: evidence of interaction with the BiP chaperone. Gyamera-Acheampong C, Sirois F, Denis NJ, Mishra P, Figeys D, Basak A, Mbikay M. Mol Cell Biochem. 2011 Feb;348(1-2):43-52. Epub 2010 Nov 16.
	Lipidomics era: accomplishments and challenges. Bou Khalil M, Hou W, Zhou H, Elisma F, Swayne LA, Blanchard AP, Yao Z, Bennett SA, Figeys D. Mass Spectrom Rev. 2010 Nov-Dec;29(6):877-929.PMID: 20931646
	Lipin - The Bridge between Hepatic Glycerolipid Biosynthesis and Lipoprotein Metabolism., Khalil MB, Blais A, Figeys D, Yao Z., Biochim Biophys Acta. 2010 Aug 5. PMID: 20692363
	Rare cell proteomic reactor applied to SILAC based quantitative proteomic study of human embryonic stem cell differentiation, Tian R, Wang S, Elisma F, Li L, Zhou H, Wang L, Figeys D., Mol Cell Proteomics. 2010 Jun 8. PMID: 20530636
	New ammunition for the proteomic reactor: strong anion exchange beads and multiple enzymes enhance protein identification and sequence coverage., Zhou H, Hou W, Lambert JP, Figeys D., Anal Bioanal Chem. 2010 Aug;397(8):3421-30. Epub 2010 Jun 3.PMID: 20517600
	Phospholipase C activity affinity purifies with the Torpedo nicotinic acetylcholine receptor., Labriola JM, Dacosta CJ, Wang S, Figeys D, Smith JC, Sturgeon RM, Baenziger JE, J Biol Chem. 2010 Apr 2;285(14):10337-43. Epub 2010 Feb 4.PMID: 20133947

	Functional analysis of the missense APOC3 mutation Ala23Thr associated with human hypotriglyceridemia., Sundaram M, Zhong S, Bou Khalil M, Zhou H, Jiang ZG, Zhao Y, Iqbal J, Hussain MM, Figeys D, Wang Y, Yao Z., J Lipid Res. 2010 Jan 23. [Epub ahead of print]PMID: 20097930

	Analysis of low-abundance proteins using the proteomic reactor with pH fractionation., Zhou H, Hou W, Lambert JP, Tian R, Figeys D., Talanta. 2010 Feb 15;80(4):1526-31. Epub 2009 Jul 14.PMID: 20082810

	Analysis of the subcellular phosphoproteome using a novel phosphoproteomic reactor., Zhou H, Elisma F, Denis NJ, Wright TG, Tian R, Zhou H, Hou W, Zou H, Figeys D., J Proteome Res. 2010 Mar 5;9(3):1279-88.PMID: 20067319

	Nonsynonymous mutations within APOB in human familial hypobetalipoproteinemia: evidence for feedback inhibition of lipogenesis and postendoplasmic reticulum degradation of apolipoprotein B., Zhong S, Magnolo AL, Sundaram M, Zhou H, Yao EF, Di Leo E, Loria P, Wang S, Bamji-Mirza M, Wang L, McKnight CJ, Figeys D, Wang Y, Tarugi P, Yao Z., J Biol Chem. 2010 Feb 26;285(9):6453-64. Epub 2009 Dec 23.PMID: 20032471

	Of proteins and DNA--proteomic role in the field of chromatin research., Lambert JP, Baetz K, Figeys D., Mol Biosyst. 2010 Jan;6(1):30-7. Epub 2009 Sep 3. Review.PMID: 20024064

	Amyloid-{beta}42 signals tau hyperphosphorylation and compromises neuronal viability by disrupting alkylacylglycerophosphocholine metabolism., Ryan SD, Whitehead SN, Swayne LA, Moffat TC, Hou W, Ethier M, Bourgeois AJ, Rashidian J, Blanchard AP, Fraser PE, Park DS, Figeys D, Bennett SA., Proc Natl Acad Sci U S A. 2009 Nov 19. [Epub ahead of print]PMID: 19926863

	Two-color cell array screen reveals interdependent roles for histone chaperones and a chromatin boundary regulator in histone gene repression., Fillingham J, Kainth P, Lambert JP, van Bakel H, Tsui K, Peña-Castillo L, Nislow C, Figeys D, Hughes TR, Greenblatt J, Andrews BJ., Mol Cell. 2009 Aug 14;35(3):340-51.PMID: 19683497

	Proteomics: from technology developments to biological applications., Abu-Farha M, Elisma F, Zhou H, Tian R, Zhou H, Asmer MS, Figeys D., Anal Chem. 2009 Jun 15;81(12):4585-99. Review. No abstract available. PMID: 19371061

	PCSK4-null sperm display enhanced protein tyrosine phosphorylation and ADAM2 proteolytic processing during in vitro capacitation., Gyamera-Acheampong C, Vasilescu J, Figeys D, Mbikay M., Fertil Steril. 2010 Mar 1;93(4):1112-23. Epub 2009 Apr 1.PMID: 19342015

	A novel proteomics approach for the discovery of chromatin-associated protein networks., Lambert JP, Mitchell L, Rudner A, Baetz K, Figeys D., Mol Cell Proteomics. 2009 Apr;8(4):870-82. Epub 2008 Dec 22.PMID: 19106085

	Glycoproteomic reactor for human plasma., Zhou H, Hou W, Denis NJ, Zhou H, Vasilescu J, Zou H, Figeys D., J Proteome Res. 2009 Feb;8(2):556-66.PMID: 19067587

	Identification of lysines within membrane-anchored Mga2p120 that are targets of Rsp5p ubiquitination and mediate mobilization of tethered Mga2p90., Bhattacharya S, Shcherbik N, Vasilescu J, Smith JC, Figeys D, Haines DS., J Mol Biol. 2009 Jan 23;385(3):718-25. Epub 2008 Nov 24.PMID: 19061897

	Identification of lysophosphatidylcholine (LPC) and platelet activating factor (PAF) from PC12 cells and mouse cortex using liquid chromatography/multi-stage mass spectrometry (LC/MS3)., Smith JC, Hou W, Whitehead SN, Ethier M, Bennett SA, Figeys D., Rapid Commun Mass Spectrom. 2008 Nov;22(22):3579-87.PMID: 18937225

	Differential proteomic screen to evidence proteins ubiquitinated upon mitotic exit in cell-free extract of Xenopus laevis embryos., Bazile F, Gagné JP, Mercier G, Lo KS, Pascal A, Vasilescu J, Figeys D, Poirier GG, Kubiak JZ, Chesnel F., J Proteome Res. 2008 Nov;7(11):4701-14. Epub 2008 Sep 30.PMID: 18823142

	Differential regulation of wild-type and mutant alpha-synuclein binding to synaptic membranes by cytosolic factors., Wislet-Gendebien S, Visanji NP, Whitehead SN, Marsilio D, Hou W, Figeys D, Fraser PE, Bennett SA, Tandon A., BMC Neurosci. 2008 Sep 22;9:92.

	Technological developments in lipidomics., Hou W, Zhou H, Elisma F, Bennett SA, Figeys D., Brief Funct Genomic Proteomic. 2008 Sep;7(5):395-409. Epub 2008 Sep 19.

	Mapping the human protein interactome., Figeys D., Cell Res. 2008 Jul;18(7):716-24. Review.PMID: 18574500

	Recent developments in mass spectrometry-based quantitative phosphoproteomics., Smith JC, Figeys D., Biochem Cell Biol. 2008 Apr;86(2):137-48. Review.PMID: 18443627

	Human Proteinpedia enables sharing of human protein data., Mathivanan S, Ahmed M, Ahn NG, Alexandre H, Amanchy R, Andrews PC, Bader JS, Balgley BM, Bantscheff M, Bennett KL, Björling E, Blagoev B, Bose R, Brahmachari SK, Burlingame AS, Bustelo XR, Cagney G, Cantin GT, Cardasis HL, Celis JE, Chaerkady R, Chu F, Cole PA, Costello CE, Cotter RJ, Crockett D, DeLany JP, De Marzo AM, DeSouza LV, Deutsch EW, Dransfield E, Drewes G, Droit A, Dunn MJ, Elenitoba-Johnson K, Ewing RM, Van Eyk J, Faca V, Falkner J, Fang X, Fenselau C, Figeys D, Gagné P, Gelfi C, Gevaert K, Gimble JM, Gnad F, Goel R, Gromov P, Hanash SM, Hancock WS, Harsha HC, Hart G, Hays F, He F, Hebbar P, Helsens K, Hermeking H, Hide W, Hjernø K, Hochstrasser DF, Hofmann O, Horn DM, Hruban RH, Ibarrola N, James P, Jensen ON, Jensen PH, Jung P, Kandasamy K, Kheterpal I, Kikuno RF, Korf U, Körner R, Kuster B, Kwon MS, Lee HJ, Lee YJ, Lefevre M, Lehvaslaiho M, Lescuyer P, Levander F, Lim MS, Löbke C, Loo JA, Mann M, Martens L, Martinez-Heredia J, McComb M, McRedmond J, Mehrle A, Menon R, Miller CA, Mischak H, Mohan SS, Mohmood R, Molina H, Moran MF, Morgan JD, Moritz R, Morzel M, Muddiman DC, Nalli A, Navarro JD, Neubert TA, Ohara O, Oliva R, Omenn GS, Oyama M, Paik YK, Pennington K, Pepperkok R, Periaswamy B, Petricoin EF, Poirier GG, Prasad TS, Purvine SO, Rahiman BA, Ramachandran P, Ramachandra YL, Rice RH, Rick J, Ronnholm RH, Salonen J, Sanchez JC, Sayd T, Seshi B, Shankari K, Sheng SJ, Shetty V, Shivakumar K, Simpson RJ, Sirdeshmukh R, Siu KW, Smith JC, Smith RD, States DJ, Sugano S, Sullivan M, Superti-Furga G, Takatalo M, Thongboonkerd V, Trinidad JC, Uhlen M, Vandekerckhove J, Vasilescu J, Veenstra TD, Vidal-Taboada JM, Vihinen M, Wait R, Wang X, Wiemann S, Wu B, Xu T, Yates JR, Zhong J, Zhou M, Zhu Y, Zurbig P, Pandey A., Nat Biotechnol. 2008 Feb;26(2):164-7. No abstract available. PMID: 18259167

	Identification of protein-protein interactions by mass spectrometry coupled techniques., Abu-Farha M, Elisma F, Figeys D., Adv Biochem Eng Biotechnol. 2008;110:67-80. Review.PMID: 18227982

	Functional dissection of the NuA4 histone acetyltransferase reveals its role as a genetic hub and that Eaf1 is essential for complex integrity., Mitchell L, Lambert JP, Gerdes M, Al-Madhoun AS, Skerjanc IS, Figeys D, Baetz K., Mol Cell Biol. 2008 Apr;28(7):2244-56. Epub 2008 Jan 22.PMID: 18212056

	The tale of two domains: proteomics and genomics analysis of SMYD2, a new histone methyltransferase., Abu-Farha M, Lambert JP, Al-Madhoun AS, Elisma F, Skerjanc IS, Figeys D., Mol Cell Proteomics. 2008 Mar;7(3):560-72. Epub 2007 Dec 7.PMID: 18065756

Systematic determination of ion score cutoffs based on calculated false positive rates: application for identifying ubiquitinated proteins by tandem mass spectrometry.

Vasilescu J, Smith JC, Zweitzig DR, Denis NJ, Haines DS, Figeys D.

J Mass Spectrom. 2008 Mar;43(3):296-304.PMID: 17957819

Identification and quantitation of changes in the platelet activating factor family of glycerophospholipids over the course of neuronal differentiation by high-performance liquid chromatography electrospray ionization tandem mass spectrometry.

Whitehead SN, Hou W, Ethier M, Smith JC, Bourgeois A, Denis R, Bennett SA, Figeys D.

Anal Chem. 2007 Nov 15;79(22):8539-48. Epub 2007 Oct 20.PMID: 17949058

Activation of the Cdc42p GTPase by cyclin-dependent protein kinases in budding yeast.

Sopko R, Huang D, Smith JC, Figeys D, Andrews BJ.

EMBO J. 2007 Oct 31;26(21):4487-500. Epub 2007 Sep 13.PMID: 17853895

Analysis of protein interaction networks using mass spectrometry compatible techniques.

Ethier M, Lambert JP, Vasilescu J, Figeys D.

Anal Chim Acta. 2006 Mar 30;564(1):10-8. Epub 2006 Jan 23.PMID: 17723357

A differential phosphoproteomic analysis of retinoic acid-treated P19 cells.

Smith JC, Duchesne MA, Tozzi P, Ethier M, Figeys D.

J Proteome Res. 2007 Aug;6(8):3174-86. Epub 2007 Jul 10.PMID: 17622165

Proteomics in 2005/2006: developments, applications and challenges.

Smith JC, Lambert JP, Elisma F, Figeys D.

Anal Chem. 2007 Jun 15;79(12):4325-43. Epub 2007 May 4. Review. PMID: 17477510

Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry.

Denis NJ, Vasilescu J, Lambert JP, Smith JC, Figeys D.

Proteomics. 2007 Mar;7(6):868-74.PMID: 17370265

Large-scale mapping of human protein-protein interactions by mass spectrometry.

Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D.

Mol Syst Biol. 2007;3:89. Epub 2007 Mar 13.PMID: 17353931

The proteomic reactor facilitates the analysis of affinity-purified proteins by mass spectrometry: application for identifying ubiquitinated proteins in human cells.

Vasilescu J, Zweitzig DR, Denis NJ, Smith JC, Ethier M, Haines DS, Figeys D.

J Proteome Res. 2007 Jan;6(1):298-305.PMID: 17203973

Multiplexed proteomic reactor for the processing of proteomic samples.

Hou W, Ethier M, Smith JC, Sheng Y, Figeys D.

Anal Chem. 2007 Jan 1;79(1):39-44.PMID: 17194119

Biomarker assay translation from discovery to clinical studies in cancer drug development: quantification of emerging protein biomarkers.

Lee JW, Figeys D, Vasilescu J.

Adv Cancer Res. 2007;96:269-98. Review.PMID: 17161683

The proteomic reactor: a microfluidic device for processing minute amounts of protein prior to mass spectrometry analysis.

Ethier M, Hou W, Duewel HS, Figeys D.

J Proteome Res. 2006 Oct;5(10):2754-9.PMID: 17022646

Proteomics technology in systems biology.

Smith JC, Figeys D.

Mol Biosyst. 2006 Aug;2(8):364-70. Epub 2006 Jun 27. Review.PMID: 16880956

Mapping protein-protein interactions by mass spectrometry.

Vasilescu J, Figeys D.

Curr Opin Biotechnol. 2006 Aug;17(4):394-9. Epub 2006 Jul 5. Review.PMID: 16822661

N-glycosylation analysis using the StrOligo algorithm.

Ethier M, Figeys D, Perreault H.

Methods Mol Biol. 2006;328:187-97.PMID: 16785650

Strategy to design improved proteomic experiments based on statistical analyses of the chemical properties of identified peptides.

Ethier M, Figeys D.

J Proteome Res. 2005 Nov-Dec;4(6):2201-6.PMID: 16335967

Proteomic analysis of ubiquitinated proteins from human MCF-7 breast cancer cells by immunoaffinity purification and mass spectrometry.

Vasilescu J, Smith JC, Ethier M, Figeys D.

J Proteome Res. 2005 Nov-Dec;4(6):2192-200.PMID: 16335966

Proteomics: from gel based to gel free.

Lambert JP, Ethier M, Smith JC, Figeys D.

Anal Chem. 2005 Jun 15;77(12):3771-87. Review. No abstract available. PMID: 15952756

Mapping protein-protein interactions.

Figeys D.

Methods Biochem Anal. 2005;45:63-84. Review. No abstract available. PMID: 19235291

Proteomics: the basic overview.

Figeys D.

Methods Biochem Anal. 2005;45:1-62. Review. No abstract available. PMID: 19235290

Definition and characterization of a "trypsinosome" from specific peptide characteristics by nano-HPLC-MS/MS and in silico analysis of complex protein mixtures.

Le Bihan T, Robinson MD, Stewart II, Figeys D.

J Proteome Res. 2004 Nov-Dec;3(6):1138-48.PMID: 15595722

The reproducible acquisition of comparative liquid chromatography/tandem mass spectrometry data from complex biological samples.

Stewart II, Zhao L, Le Bihan T, Larsen B, Scozzaro S, Figeys D, Mao GD, Ornatsky O, Dharsee M, Orsi C, Ewing R, Goh T.

Rapid Commun Mass Spectrom. 2004;18(15):1697-710.PMID: 15282768

Combining different 'omics' technologies to map and validate protein-protein interactions in humans.

Figeys D.

Brief Funct Genomic Proteomic. 2004 Feb;2(4):357-65. Review.PMID: 15163370

HysTag--a novel proteomic quantification tool applied to differential display analysis of membrane proteins from distinct areas of mouse brain.

Olsen JV, Andersen JR, Nielsen PA, Nielsen ML, Figeys D, Mann M, Wisniewski JR.

Mol Cell Proteomics. 2004 Jan;3(1):82-92. Epub 2003 Nov 10.PMID: 14610161

Proteomics in 2002: a year of technical development and wide-ranging applications.

Figeys D.

Anal Chem. 2003 Jun 15;75(12):2891-905. Review. No abstract available. PMID: 12945794

On-line strong cation exchange micro-HPLC-ESI-MS/MS for protein identification and process optimization.

Le Bihan T, Duewel HS, Figeys D.

J Am Soc Mass Spectrom. 2003 Jul;14(7):719-27.PMID: 12837593

Novel approaches to map protein interactions.

Figeys D.

Curr Opin Biotechnol. 2003 Feb;14(1):119-25. Review.PMID: 12566011

Death-associated protein 4 binds MST1 and augments MST1-induced apoptosis.

Lin Y, Khokhlatchev A, Figeys D, Avruch J.

J Biol Chem. 2002 Dec 13;277(50):47991-8001. Epub 2002 Oct 15.PMID: 12384512

Proteomics approaches in drug discovery.

Figeys D.

Anal Chem. 2002 Aug 1;74(15):412A-419A. Review. No abstract available. PMID: 12175190

Adapting arrays and lab-on-a-chip technology for proteomics.

Figeys D.

Proteomics. 2002 Apr;2(4):373-82. Review.PMID: 12164696

Functional proteomics: mapping protein-protein interactions and pathways.

Figeys D.

Curr Opin Mol Ther. 2002 Jun;4(3):210-5. Review.PMID: 12139305

Finding the 'lost' genes.

Figeys D, Rossetto F.

Trends Biotechnol. 2002 Apr;20(4):144. No abstract available. PMID: 11906742

Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry.

Ho Y, Gruhler A, Heilbut A, Bader GD, Moore L, Adams SL, Millar A, Taylor P, Bennett K, Boutilier K, Yang L, Wolting C, Donaldson I, Schandorff S, Shewnarane J, Vo M, Taggart J, Goudreault M, Muskat B, Alfarano C, Dewar D, Lin Z, Michalickova K, Willems AR, Sassi H, Nielsen PA, Rasmussen KJ, Andersen JR, Johansen LE, Hansen LH, Jespersen H, Podtelejnikov A, Nielsen E, Crawford J, Poulsen V, Sørensen BD, Matthiesen J, Hendrickson RC, Gleeson F, Pawson T, Moran MF, Durocher D, Mann M, Hogue CW, Figeys D, Tyers M.

Nature. 2002 Jan 10;415(6868):180-3.PMID: 11805837

18O labeling: a tool for proteomics.

Stewart II, Thomson T, Figeys D.

Rapid Commun Mass Spectrom. 2001;15(24):2456-65.PMID: 11746917

Mass spectrometry for the study of protein-protein interactions.

Figeys D, McBroom LD, Moran MF.

Methods. 2001 Jul;24(3):230-9. Review.PMID: 11403572

Nanoflow gradient generator coupled with mu-LC-ESI-MS/MS for protein identification.

Le Bihan T, Pinto D, Figeys D.

Anal Chem. 2001 Mar 15;73(6):1307-15.PMID: 11305668

Proteomics on a chip: promising developments.

Figeys D, Pinto D.

Electrophoresis. 2001 Jan;22(2):208-16. Review.PMID: 11288886

Ras binding triggers ubiquitination of the Ras exchange factor Ras-GRF2.

de Hoog CL, Koehler JA, Goldstein MD, Taylor P, Figeys D, Moran MF.

Mol Cell Biol. 2001 Mar;21(6):2107-17.PMID: 11238945

Web surfing in yeast: a network of proteins.

Figeys D.

Trends Biotechnol. 2001 Mar;19(3):85-6. No abstract available. PMID: 11179784

The Achilles' heel of proteomics.

Figeys D.

Trends Biotechnol. 2000 Dec;18(12):483. No abstract available. PMID: 11102657

Large-scale screening on small scale.

Figeys D.

Trends Biotechnol. 2000 Sep;18(9):363-4. No abstract available. PMID: 10942958

Techniques for the optimization of proteomic strategies based on head column stacking capillary electrophoresis.

Locke S, Figeys D.

Anal Chem. 2000 Jul 1;72(13):2684-9.PMID: 10905293

Lab-on-a-chip: a revolution in biological and medical sciences

Figeys D, Pinto D.

Anal Chem. 2000 May 1;72(9):330A-335A. No abstract available. PMID: 10815945

An enhanced microfluidic chip coupled to an electrospray Qstar mass spectrometer for protein identification.

Pinto DM, Ning Y, Figeys D.

Electrophoresis. 2000 Jan;21(1):181-90.PMID: 10634486

Array and lab on a chip technology for protein characterization.

Figeys D.

Curr Opin Mol Ther. 1999 Dec;1(6):685-94. Review.PMID: 19629865

Identification of flow-dependent endothelial nitric-oxide synthase phosphorylation sites by mass spectrometry and regulation of phosphorylation and nitric oxide production by the phosphatidylinositol 3-kinase inhibitor LY294002.

Gallis B, Corthals GL, Goodlett DR, Ueba H, Kim F, Presnell SR, Figeys D, Harrison DG, Berk BC, Aebersold R, Corson MA.

J Biol Chem. 1999 Oct 15;274(42):30101-8.PMID: 10514497

Data-dependent modulation of solid-phase extraction capillary electrophoresis for the analysis of complex peptide and phosphopeptide mixtures by tandem mass spectrometry: application to endothelial nitric oxide synthase.

Figeys D, Corthals GL, Gallis B, Goodlett DR, Ducret A, Corson MA, Aebersold R.

Anal Chem. 1999 Jul 1;71(13):2279-87.PMID: 10405598

Microfabricated modules for sample handling, sample concentration and flow mixing: application to protein analysis by tandem mass spectrometry.

Figeys D, Aebersold R.

J Biomech Eng. 1999 Feb;121(1):7-12.PMID: 10080083

Microfabricated device coupled with an electrospray ionization quadrupole time-of-flight mass spectrometer: protein identifications based on enhanced-resolution mass spectrometry and tandem mass spectrometry data.

Figeys D, Lock C, Taylor L, Aebersold R.

Rapid Commun Mass Spectrom. 1998;12(20):1435-44.PMID: 9796531

Optimization of solid phase microextraction - capillary zone electrophoresis - mass spectrometry for high sensitivity protein identification.

Figeys D, Zhang Y, Aebersold R.

Electrophoresis. 1998 Oct;19(13):2338-47.PMID: 9788318

An integrated microfluidics-tandem mass spectrometry system for automated protein analysis.

Figeys D, Gygi SP, McKinnon G, Aebersold R.

Anal Chem. 1998 Sep 15;70(18):3728-34.PMID: 9751017

Nanoflow solvent gradient delivery from a microfabricated device for protein identifications by electrospray ionization mass spectrometry.

Figeys D, Aebersold R.

Anal Chem. 1998 Sep 15;70(18):3721-7.PMID: 9751016

Proteome analysis: biological assay or data archive?

Haynes PA, Gygi SP, Figeys D, Aebersold R.

Electrophoresis. 1998 Aug;19(11):1862-71. Review.PMID: 9740046

Towards an integrated analytical technology for the generation of multidimensional protein expression maps.

Aebersold R, Figeys D, Gygi S, Corthals G, Haynes P, Rist B, Sherman J, Zhang Y, Goodlett D.

J Protein Chem. 1998 Aug;17(6):533-5. No abstract available. PMID: 9723742

Electrophoresis combined with novel mass spectrometry techniques: powerful tools for the analysis of proteins and proteomes.

Figeys D, Gygi SP, Zhang Y, Watts J, Gu M, Aebersold R.

Electrophoresis. 1998 Jul;19(10):1811-8.PMID: 9719564

Purification of trypsin for mass spectrometric identification of proteins at high sensitivity.

Zhang Y, Figeys D, Aebersold R.

Anal Biochem. 1998 Jul 15;261(1):124-7. No abstract available. PMID: 9683523

High sensitivity analysis of proteins and peptides by capillary electrophoresis-tandem mass spectrometry: recent developments in technology and applications.

Figeys D, Aebersold R.

Electrophoresis. 1998 May;19(6):885-92. Review.PMID: 9638934

A microfabricated device for rapid protein identification by microelectrospray ion trap mass spectrometry.

Figeys D, Ning Y, Aebersold R.

Anal Chem. 1997 Aug 15;69(16):3153-60.PMID: 9271059

Capillary electrophoresis of peptides and proteins at neutral pH in capillaries covalently coated with polyethyleneimine.

Figeys D, Aebersold R.

J Chromatogr B Biomed Sci Appl. 1997 Jul 18;695(1):163-8.PMID: 9271140

High sensitivity identification of proteins by electrospray ionization tandem mass spectrometry: initial comparison between an ion trap mass spectrometer and a triple quadrupole mass spectrometer.

Figeys D, Aebersold R.

Electrophoresis. 1997 Mar-Apr;18(3-4):360-8.PMID: 9150914

Identification of proteins by capillary electrophoresis-tandem mass spectrometry. Evaluation of an on-line solid-phase extraction device.

Figeys D, Ducret A, Aebersold R.

J Chromatogr A. 1997 Feb 28;763(1-2):295-306.PMID: 9129327

Protein identification by solid phase microextraction-capillary zone electrophoresis-microelectrospray-tandem mass spectrometry.

Figeys D, Ducret A, Yates JR 3rd, Aebersold R.

Nat Biotechnol. 1996 Nov;14(11):1579-83.PMID: 9634825

Pseudo-coulometric loading in capillary electrophoresis DNA sequencing.

Figeys D, Ahmadzedeh H, Arriaga E, Dovichi NJ.

J Chromatogr A. 1996 Sep 13;744(1-2):325-31.PMID: 8843681

Protein identification by capillary zone electrophoresis/microelectrospray ionization-tandem mass spectrometry at the subfemtomole level.

Figeys D, van Oostveen I, Ducret A, Aebersold R.

Anal Chem. 1996 Jun 1;68(11):1822-8.PMID: 8686909

Labeling of double-stranded DNA by ROX-dideoxycytosine triphosphate using terminal deoxynucleotidyl transferase and separation by capillary electrophoresis.

Figeys D, Renborg A, Dovichi NJ.

Anal Chem. 1994 Dec 1;66(23):4382-3.PMID: 7847633

Spatial and temporal depletion of ions from noncrosslinked denaturing polyacrylamide in capillary electrophoresis.

Figeys D, Renborg A, Dovichi NJ.

Electrophoresis. 1994 Dec;15(12):1512-7.PMID: 7720687

Activation energy of single-stranded DNA moving through cross-linked polyacrylamide gels at 300 V/cm. Effect of temperature on sequencing rate in high-electric-field capillary gel electrophoresis.

Lu H, Arriaga E, Chen DY, Figeys D, Dovichi NJ.

J Chromatogr A. 1994 Oct 7;680(2):503-10.PMID: 7981831