Speaker: Lynn Megeney Mach Gaennelsen Chair in Cardiac Research, Molecular Medicine Program, Centre for Stem Cell Research, Ottawa Health Research Institute and the University of Ottawa. Moving Toward Functional Phosphoproteomics. Measuring and Inferring Global Kinase Activity Patterns Phosphorylation is the most prevalent post-translational protein modification identified to date. Given the central importance of this modification in the life cycle of most proteins, a systems wide annotation of phosphorylation events has become a common strategy for identifying probable regulatory responses in a variety of organisms. Despite the success of this approach, little effort has been directed toward global assessment of the enzymes which regulate the phosphorylation process itself, the kinase protein superfamily. Here we discuss our efforts to employ a systems biology paradigm to identify global kinase activity patterns. This approach entails the development of proteomic screening tools in which kinase activity profiles can be coupled with mass spectrometry identification of the kinases in question. We will also discuss our efforts to combine phosphoproteome data with bioinformatics tools as a means to infer or model kinase activity for specific biologic models.